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Beilstein J. Org. Chem. 2014, 10, 2470–2479, doi:10.3762/bjoc.10.258
Graphical Abstract
Figure 1: Structure of the reporter molecule BPT (1).
Scheme 1: Synthesis of the azide-bearing 4-hydroxythiazole derivative 1.
Figure 2: Structure of the tested azide-modified standard fluorophores DNS (8) and NBD (9) and the bromine mo...
Figure 3: UV–vis spectra of 20 µM solutions of the azide modified fluorophores BPT (1), DNS (8), NBD (9) and ...
Figure 4: Normalized absorbance and fluorescence of BPT (1) in 20% THF/80% water (v/v), excitation at 374 nm.
Figure 5: Peak area of 100 pmol BPT (1), DNS (8), NBD (9) and BNS (6) measured with (A) C18-UPLC coupled to a...
Figure 6: Procedure of the model reaction between L-lysine and DDY (10) to form an imine (only one of two pos...
Figure 7: Mass spectra of labeled L-lysine/DDY (10)/fluorophore conjugates 11 (containing BPT), 12 (containin...
Figure 8: Fluorescent labeling of catalase treated with DDY (10) followed by CuAAC with all four reporter mol...